Separation of Lactate Dehydrogenase Isozymes from Various Mouse Tissues.

Isozymes of lactic dehydrogenase in human tissues.

The heterogeneity of lactic dehydrogenase (LDH) activity in human serum and erythrocytes has been observed in several laboratories (1-7). If each of the electrophoretically-distinguishable enzymes in serum with LDH activity were derived from different tissues, in disease states involving a single organ the normal pattern of serum LDH activity would be altered according to the type of LDH releas...

Isozymes of lactate dehydrogenase (LDH) in skeletal tissues of the embryonic and newly hatched chick.

Amino- and carboxyl-terminal analyses of hepatoma lactate dehydrogenase isozymes.

The M4 isozyme of lactate dehydrogenase was purified to homogeneity from normal rat liver and from two Morris hepatomas (7777 and 7793). Amino-terminal analyses with fluorodinitrobenzene failed to detect the presence of free amino-terminal residues in each enzyme studied. Each enzyme contained between 3.7 and 4.1 moles of protein-bound acetyl groups per mole of enzyme. The amino-terminal peptid...

Genetic Evidence for the Subunit Structure of Lactate Dehydrogenase Isozymes.

The lactate dehydrogenases commonly occur in from one to five molecular forms (isozymes) in the various tissues of an animal species.' By means of zone electrophoresis, characteristic patterns of these isozymes may be demonstrated in each tissue. In adult animals, most tissues contain all five isozymes but in different proportions. Usually, heart muscle exhibits principally the first, or most n...

[Lactate dehydrogenase in human oral neoplastic tissues].

要 旨:エ ナメル上皮腫,歯 肉癌 および唾液腺腫瘍 の各種 口腔領 域上皮性腫瘍組織 につ いて,DNA量 あた りのLDH活 性 とLDH isoenzyme patternを 正常対 照組織 と比較 して検討 した。 LDH活 性 は,Wroblewski and LaDueの 方 法に準 じてNADHの 吸光度変化 に よ り測 定 した。LDH isoenzyme patternは,Dietz and Lubranoの 方 法に準 じてdisc電 気泳動 によ り分離 し,Ressler氏 法の 変法 による染色液 で酵素活性 を検 出 しdensitometryに よ り求 めた。 その結果,一 般 に腫瘍組織 においてはLDH活 性の上昇が認 め られ た。 エナメル上皮腫 のLDHisoenzyme patternはLDH-3にpeakが み られ,仔 牛 エナメル器...